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HelpTest Code DLL3 Delta-Like 3 Protein (SP347), Semi-Quantitative Immunohistochemistry, Manual, Tissue
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Necessary Information
A pathology/diagnostic report and a brief history are required.
Specimen Required
Specimen Type: Tissue
Supplies:
-Pathology Packaging Kit (T554)
-Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
Collection Instructions: Formalin-fixed, paraffin-embedded tissue block; or 3 unstained glass, “positively charged†slides with 4-microns, formalin-fixed, paraffin-embedded tissue
Additional Information: One slide will be stained with hematoxylin and eosin and returned.
Secondary ID
603332Useful For
Diagnosis of small cell lung carcinoma (SCLC), large cell neuroendocrine carcinoma (LCNEC), amongst other tumors
Method Name
Immunohistochemistry (IHC)
Reporting Name
DLL3(SP347)Semi-Quant IHC,ManualSpecimen Type
SpecialSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Special | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides |
Reject |
Clinical Information
Delta-like 3 protein (DLL3) is an atypical Notch ligand induced by the neuroendocrine transcription factor, ASCL-1. DLL3 is expressed on the cell membrane and in the cytoplasm of tumor cells.Â
Interpretation
Positivity for this test will require 50% or more of the tumor cells within a specimen to express delta-like 3 protein with an intensity ranging from 1 to 3+.
Cautions
This test has been validated for non-decalcified paraffin embedded tissue specimens fixed in 10% neutral buffered formalin. This assay has not been validated on tissues subjected to the decalcification process and/or use of alternative fixatives for bone/bone marrow specimens or cell blocks.
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks.
Clinical Reference
1. Saunders LR, Bankovich AJ, Anderson WC, et al. A DLL3-targeted antibody-drug conjugate eradicates high-grade pulmonary neuroendocrine tumor-initiating cells in vivo. Sci Transl Med. 2015;7(302):302ra136
2. Xie H, Boland JM, Maleszewski JJ, et al. Expression of delta-like protein 3 is reproducibly present in a subset of small cell lung carcinomas and pulmonary carcinoid tumors. Lung Cancer. 2019;135:73-79. doi:10.1016/j.lungcan.2019.07.016
3. Xie H, Kaye FJ, Isse K, et al. Delta-like protein 3 expression and targeting in merkel cell carcinoma. Oncologist. 2020;10:810-817. doi:10.1634/theoncologist.2019-0877
4. Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. Methods Mol Biol. 2019;1897:289-298. doi:10.1007/978-1-4939-8935-5_25
Method Description
Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Report Available
5 to 7 daysSpecimen Retention Time
Until reportedPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88360
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DLL3 | DLL3(SP347)Semi-Quant IHC,Manual | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 603333 | Interpretation | 59465-5 |
| 603334 | Participated in the Interpretation | No LOINC Needed |
| 603335 | Report electronically signed by | 19139-5 |
| 603336 | Material Received | 81178-6 |
| 603337 | Disclaimer | 62364-5 |
| 603338 | Case Number | 80398-1 |
Forms
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.