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Test Code EXHR Hematologic Disorders, DNA and RNA Extract and Hold, Varies

Secondary ID

65114

Useful For

Reserving nucleic acids on any specimen for which molecular analysis requiring DNA or RNA may be necessary at a future date, ensuring that adequate material for testing is available

Testing Algorithm

DNA and RNA will be extracted from the specimen and stored at -80° Celsius for one year from the date the extraction process was performed. A report of "Performed" will be sent and a $75.00 processing fee will be assessed. To request molecular testing from the Molecular Hematopathology Laboratory's test menu, call 800-533-1710. Additional charges will be assessed for testing performed.

 

For more information see Eosinophilia: Bone Marrow Diagnostic Algorithm.

Method Name

Nucleic Acid Extraction

Reporting Name

DNA and RNA Extract and Hold

Specimen Type

Varies


Shipping Instructions


Refrigerate specimens must arrive within 5 days of collection, and ambient specimens must arrive with 3 days (72 hours) of collection. Collect and package specimens as close to shipping time as possible.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 10 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Label specimen as blood.

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send bone marrow specimen in original tube. Do not aliquot.

3. Label specimen as bone marrow.


Specimen Minimum Volume

Blood: 8 mL
Bone Marrow: 2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 5 days PURPLE OR PINK TOP/EDTA
  Ambient  72 hours PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Paraffin-embedded material
Fluids
Heparinized samples		 Reject

Clinical Information

It is frequently useful to obtain nucleic acids from clinical samples containing a hematopoietic neoplasm at the time of diagnosis so appropriate material is available for molecular analysis should subsequent testing be required. For example, when a diagnosis of acute myelogenous leukemia is made, there is a delay before karyotype information, which determines whether testing for molecular prognostic markers is necessary, is available. After this delay, the diagnostic sample is usually no longer available or the nucleic acid has degraded to such an extent that it is no longer adequate for testing. Thus, it is useful to obtain nucleic acids on such specimens promptly at diagnosis and retain it until it is known whether additional testing is necessary.

Reference Values

Not applicable

Interpretation

A report of "Performed" will be sent and a $75 processing fee will be assessed. No interpretation will be given. Should the sample be used in future testing, interpretation would be incorporated with the final testing.

Cautions

DNA/RNA will be held at -80° Celsius for one year from the time of extraction. The nucleic acids may only be used for suitable tests offered by the Molecular Hematopathology Laboratory at Mayo Clinic. Nucleic acid samples cannot be shipped to another institution for testing and cannot be released for research use.

 

Only liquid blood specimens will be processed. Paraffin-embedded material and fresh, solid tissue will not be accepted at this time.

Method Description

DNA and RNA are extracted using automated systems. Both systems include a sample lysis step followed by binding of nucleic acid to a solid phase for separation from other cellular components and a final nucleic acid elution step.

Day(s) Performed

Monday through Saturday

Report Available

5 days

Specimen Retention Time

Blood/Bone marrow: 2 weeks; Extracted DNA and RNA: 1 year

Performing Laboratory

Mayo Clinic Laboratories in Rochester

LOINC Code Information

Result ID Test Result Name Result LOINC Value
MP035 Specimen Type 31208-2
41938 DNA/RNA Extract and Hold Result No LOINC Needed
41937 DNA/RNA Extraction No LOINC Needed

Test Classification

Not Applicable

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.