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Test Code HK36M Histone H3 K36M Mutant (H3F3 K36M) Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

Mayo Clinic Laboratories has multiple histone immunostains available. See table for ordering guidance.

 

Test ID

Published name

Indication

Mayo Clinic slide label

HG34W

Histone 3.3 G34W (H3F3A G34W) Immunostain, Technical Component Only

Giant cell tumor of bone (GCTB)

H3 G34W

HK27M

Histone H3 K27M Mutant (H3 K27M) Immunostain, Technical Component Only

K27M mutant midline gliomas

H3 K27M

HISME

Histone H3 Trimethyl K27 (H3 K27me[3]) Immunostain, Technical Component Only

MPNST and K27M mutant midline gliomas

H3 K27me3

HK36M

Histone H3 K36M Mutant (H3F3 K36M) Immunostain, Technical Component Only

Chondroblastoma

H3 K36M


Shipping Instructions


Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Specimen Type: Tissue

Supplies: Immunostain Technical Only Envelope (T693)

Container/Tube: Immunostain Technical Only Envelope

Preferred:

-Formalin-fixed, paraffin-embedded tissue block

OR

-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: None


Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Secondary ID

604699

Useful For

Diagnosis of chondroblastoma

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOA IHC Additional, Tech Only No No
IHTOI IHC Initial, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

Histone H3 K36M IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides
Snowcoat slides		 Reject

Clinical Information

H3F3B is a member of the histone H3 family and is located on chromosome 17. Histone H3 K36M is expressed in the nuclei of the mononuclear cell population of H3F3B altered chondroblastoma.

Interpretation

This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.

 

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks.

 

The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.

 

Best practices for storage of positively charged slides:

-Minimize time slides are stored after being unpackaged

-Limit exposure to high humidity and heat

-Minimize exposure to plastics

Clinical Reference

1. Cleven A, Hocker S, Briaire-de Bruijn I, Szuhai K, Cleton-Jansen AM, Bovee JVMG. Mutation analysis of H3F3A and H3F3B as a diagnostic tool for giant cell tumor of bone and chondroblastoma. Am J Surg Pathol. 2015;39(11):1576-1583

2. Lu C, Jain S, Hoelper D, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352(6287):844-849

3. Chen W, DiFrancesco LM. Chondroblastoma: An update. Arch Pathol Lab Med. 2017;141(6):867-871

4. Amary MF, Berisha F, Mozela R, et al. The H3F3 K36M mutant antibody is a sensitive and specific marker for the diagnosis of chondroblastoma. Histopathology. 2016;69(2):121-127

5. Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. Methods Mol Biol. 2019;1897:289-298. doi:10.1007/978-1-4939-8935-5_25

Method Description

Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday 

Report Available

1 to 3 days

Specimen Retention Time

Until staining is complete

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HK36M Histone H3 K36M IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
604700 Histone H3 K36M IHC, Tech Only Bill only; no result

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).