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HelpTest Code IDCOR Red Blood Cell Antigen Genotyping, PCR, Blood
Ordering Guidance
This test is used to determine red blood cell genotype and predict the phenotype. It does not determine the A1 phenotype subtype or aid in the diagnosis of McLeod syndrome.
-If testing for McLeod syndrome (phenotype) is desired, order SPAGR / Special Red Cell Antigen Typing, Whole Blood and note "K system antigens" on the order.
-If the A1 subtype is requested, order SPAGR and note "A1 antigen" on the order.
Shipping Instructions
1. Specimen must arrive within 7 days of collection.
2. Collect and package specimen as close to shipping time as possible.
Necessary Information
Pertinent clinical history, including whether the patient has received a bone marrow transplant, is required.
Specimen Required
Container/Tube:
Preferred: Pink top (EDTA)
Acceptable: Lavender top (EDTA)
Specimen Volume: 6 mL
Pediatric Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Label specimen as blood.
3. Send whole blood specimen in the original tube. Do not aliquot.
Secondary ID
622374Useful For
Determining the red blood cell status of any patient but more useful than serology in the following situations:
-Recently transfused patient.
-Patient with a positive direct antiglobulin test.
-Patient with a discrepant antigen typing with antibodies present.
This test is not useful for the purpose of:
-Establishing paternity
-Diagnosing McLeod syndrome or determining the McLeod phenotype
-Determining A1 subtype
Highlights
This test can be used to determine red blood cell genotype and predict the red blood cell phenotype. The following red cell antigens will be displayed in the final report:
C, E, c, e, CW, V, hrS, VS, hrB, K, k, Kpa, Kpb, Jsa, Jsb, Jka, Jkb, Fya, Fyb, M, N, S, s, U, Mia, Dia, Dib, Doa, Dob, Hy, Joa, Coa, Cob, Yta, Ytb, Lua, and Lub.
Method Name
Molecular Genotyping
Reporting Name
IDCORE XT RBC GenotypeSpecimen Type
Whole Blood EDTASpecimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 7 days |
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Clinical Information
The predicted red blood cell (RBC) phenotype will determine the presence or absence of an RBC antigen. As a general rule, individuals will not make an antibody directed against an antigen present on their own RBCs.
Reference Values
The presence or absence of each red blood cell antigen is identified.
Interpretation
Each antigen will be listed by name, followed by a "+" indicating the antigen is present, or by a "0" indicating the antigen is absent. Additional footnotes may be included indicating additional information is present along with the result.
Cautions
Samples yielding low DNA concentration (<20 ng/mcL) that falls outside a DNA purity range (A260/A280) of 1.65 to 2.00 may not provide valid results. Additionally, the assay will not detect all variants of allele haplotypes.
Clinical Reference
1. Mouro I, Colin Y, Sistonen P, Le Pennec PY, Cartron JP, Le Van Kim C. Molecular basis of the RhCW (Rh8) and RhCX (Rh9) blood group specificities. Blood. 1995;86(3):1196-1201.
2. Doscher A, Vogt C, Bittner R, Gerdes I, Petershofen EK, Wagner FF. RHCE alleles detected after weak and/or discrepant results in automated Rh blood grouping of blood donors in Northern Germany. Transfusion. 2009;49(9):1803-1811. doi:10.1111/j.1537-2995.2009.02221.x
3. Doescher A, Wagner FF, Vogt C, Glameyer T, Petershofen EK. P-306: Three new polymorphisms in patients with serologic suspect of weakened expression of RHC-antigen. Vox Sang. 2005;89 (Suppl.1):159
4. Ochoa G, Nogues N, Muniz-Diaz E, et al. P-480: Association of altered E RHCE*cE(697G,712G,733G,744C) with altered D RHD*46C. Vox Sang. 2012;103 (Suppl.1):218
5. Goldman M, Cemborain A, Cote J, et al. Identification of six new RHCE variant alleles in individuals of diverse racial origin. Transfusion. 2016;56(1):244-248. doi:10.1111/trf.13357
6. Cherif-Zahar B, Raynal V, Cartron JP. Lack of RHCE-encoded proteins in the D--phenotype may result from homologous recombination between the two RH genes. Blood. 1996;88(4):1518-1520.
7. Mouro I, Colin Y, Cherif-Zahar B, Cartron JP, Le Van Kim C. Molecular genetic basis of the human Rhesus blood group system. Nat Genet. 1993;5(1):62-65. doi:10.1038/ng0993-62
8. Ochoa-Garay G, Moulds JM, Cote J, et al. New RHCE variant alleles encoding the D-?- phenotype. Transfusion. 2013;53(11 Suppl 2):3018-3023. doi:10.1111/trf.12404
9. Billingsley KL, Posadas JB, Moulds JM, Gaur LK. A novel JK null allele associated with typing discrepancies among African Americans. Immunohematology. 2013;29(4):145-148.
10. Bugert P, Scharberg EA, Geisen C, von Zabern I, Flegel WA. RhCE protein variants in Southwestern Germany detected by serologic routine testing. Transfusion. 2009;49(9):1793-1802. doi:10.1111/j.1537-2995.2009.02220.x
11. Noizat-Pirenne F, Mouro I, Gane P, et al. Heterogeneity of blood group RhE variants revealed by serological analysis and molecular alteration of the RHCE gene and transcript. Br J Haematol. 1998;103(2):429-436. doi:10.1046/j.1365-2141.1998.01004.x
12. Ekman GC, Hessner MJ. Screening of six racial groups for the intron 5 G-->A 3' splice acceptor mutation responsible for the polynesian kidd (a-b-) phenotype: the null mutation is not always associated with the JKB allele. Transfusion. 2000;40(7):888-889. doi:10.1046/j.1537-2995.2000.40070888.x
13. Zimmerman PA, Woolley I, Masinde GL, et al. Emergence of FY*A(null) in a Plasmodium vivax-endemic region of Papua New Guinea. Proc Natl Acad Sci U S A. 1999;96(24):13973-13977. doi:10.1073/pnas.96.24.13973
14. Lopez GH, Condon JA, Wilson B, et al. A novel FY*A allele with the 265T and 298A SNPs formerly associated exclusively with the FY*B allele and weak Fy(b) antigen expression: implication for genotyping interpretative algorithms. Vox Sang. 2015;108(1):52-57. doi:10.1111/vox.12185
15. Daniels G. Human Blood Groups. Wiley-Blackwell; 2013
16. Reid M, Lomas-Francis C, Olsson ML. The Blood Group Antigen Facts Book. 3rd ed. Academic Press; 2012
17. The International Society of Blood Transfusion (ISBT) Working Party for Red Cell Immunogenetics and Blood Group Terminology. Red cell immunogenetics and blood group terminology. ISBT; Revised October 2024. Accessed May 13, 2025. Available at www.isbtweb.org/working-parties/red-cell-immunogenetics-and-blood-group-terminology
18. Moller M, Joud M, Storry JR, Olsson ML. Erythrogene: a database for in-depth analysis of the extensive variation in 36 blood group systems in the 1000 Genomes Project. Blood Adv. 2016;1(3):240-249. Published 2016 Dec 16. doi:10.1182/bloodadvances.2016001867
19. RHCE Table. New York Blood Center Enterprises; 2025. Accessed May 14, 2025. Available at www.nybce.org/national-center-for-blood-group-genomics/rhce-table/
Method Description
The ID CORE XT assay utilizes Luminex xMAP technology. Genomic DNA extracted from human EDTA anticoagulated whole blood is amplified and biotinylated by multiplex polymerase chain reaction (PCR). PCR products are denatured and hybridized to oligonucleotide probes coupled to color-coded beads. Hybridized DNA is labeled with a fluorescent conjugate, and the resulting signal is detected with a Luminex 200 system. Raw data are processed with ID CORE XT analysis software to obtain benign alteration (previously polymorphism) genotypes, predicted allele genotypes, and predicted phenotypes for: C, E, c, e, CW, V, hrS, VS, hrB, K, k, Kpa, Kpb, Jsa, Jsb, Jka, Jkb, Fya, Fyb, M, N, S, s, U, Mia, Dia, Dib, Doa, Dob, Hy, Joa, Coa, Cob, Yta, Ytb, Lua, and Lub.(Package insert: ID CORE XT. Grifols; PI_1021720000_05, 01/2023)
Day(s) Performed
Monday through Friday
Report Available
2 to 7 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
0084U
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| IDCOR | IDCORE XT RBC Genotype | 93914-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 623290 | IDCORE XT RBC Genotype | 906-8 |