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HelpTest Code IL1B Interleukin-1 Beta, Plasma
Ordering Guidance
If this test is ordered with CYPAN / Cytokine Panel, Plasma, this test will be canceled as duplicate and CYPAN performed as ordered.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Immediately after specimen collection, place tube on wet ice.
2. Centrifuge at 4° C, 1500 x g for 10 minutes.
3. Aliquot plasma into plastic vial.
4. Freeze specimen within 2 hours of collection.
Additional Information:
1. Plasma for this test cannot be shared with other tests. Submit specimen for this test in its own vial.
2. This test cannot be added on to a previously collected specimen.
Secondary ID
622327Useful For
Measuring the concentration of interleukin-1 beta in plasma
Evaluating patients experiencing inflammatory conditions, autoinflammatory disorders, and infection
Highlights
This test may aid the evaluation and monitoring of patients experiencing inflammatory conditions, autoinflammatory disorders, and infection.
Method Name
Bead-Based Multiplex Immunoassay
Reporting Name
IL-1 Beta, PSpecimen Type
Plasma EDTASpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma EDTA | Frozen | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-treated specimen | Reject |
Clinical Information
Interleukin-1 (IL-1) beta is a potent pro-inflammatory cytokine that is crucial for host-defence responses to infection and injury. IL-1 beta binds to IL-1 receptor 1 (IL-1R1) and initiates inflammatory responses in T-lymphocytes, epithelial cells and endothelial cells.(1,2) Excess IL-1 beta signaling have been associated with acute inflammation and chronic autoinflammatory diseases.(3) Plasma IL-1 beta concentrations are elevated in some rheumatoid arthritis patients compared to healthy volunteers. There are increased concentrations of IL-1 beta and tumor necrosis factor-alpha in synovial fluid of rheumatoid arthritis patients.
Several biologics, such as anakinra and canakinumab, have been developed to inhibit the IL-1 signaling pathway by blocking IL-1 beta binding to IL-1 receptors in rheumatoid arthritis, familial Mediterranean fever, cryopyrin-associated periodic syndromes, etc.(2,4)
Reference Values
<18 years: Not established
≥18 years: <20.0 pg/mL
Interpretation
Elevated interleukin-1 beta concentrations could indicate the presence of inflammatory or infectious condition.
Cautions
Results from interleukin-1 (IL-1) beta testing should not be used to establish or exclude a specific diagnosis.
Testing for IL-1 beta should only be used in conjunction with clinical information and other laboratory testing as part of a patient's overall assessment.
Normal concentration of IL-1 beta does not exclude the possibility of infection or inflammatory disorders.
Concentrations of IL-1B could be affected by immunomodulatory agents.
Clinical Reference
1. Pretre V, Papadopoulos D, Regard J, Pelletier M, Woo J. Interleukin-1 (IL-1) and the inflammasome in cancer. Cytokine. 2022;153:155850
2. Imazio M, Lazaros G, Gattorno M, et al. Anti-interleukin-1 agents for pericarditis: a primer for cardiologists. Eur Heart J. 2022;43(31):2946-2957
3. Carta S, Semino C, Sitia R, Rubartelli A. Dysregulated IL-1 beta Secretion in Autoinflammatory Diseases: A Matter of Stress?. Front Immunol. 2017;8:345
4. Kaneko N, Kurata M, Yamamoto T, Morikawa S, Masumoto J. The role of interleukin-1 in general pathology. Inflamm Regen. 2019;39:12
Method Description
Testing for interleukin-1 beta levels is performed using a laboratory-developed bead-based immunoassay.(Unpublished Mayo method)
Day(s) Performed
Tuesday
Report Available
2 to 8 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
83520
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| IL1B | IL-1 Beta, P | 13629-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 622327 | IL-1 beta | 13629-1 |