Test Code LAB4004 CORTISOL ACTH STIMULATION (BASELINE, 30MIN, 60MIN)
Specimen Type
Preferred Sample: Lithium Heparin Plasma (PST, Mint Green Tube)
Alternate Sample: Serum (SST, Gold, Corvac, Tiger, Red Top Tube)
Collection Instructions
The baseline cortisol specimen should be collected prior the administration of the cosyntropin medication.
The 30 min cortisol sample should be collected 30 minutes after the administration of the cosyntropin medication.
The 60 min cortisol sample should be collected 60 minutes after the administration of the cosyntropin medication.
See also: Cortisol Collection Job Aid in PPM
Specimen Volume
1 mL for each timed sample
Minimum Volume
0.5 mL for each timed sample
Turnaround Time
STAT: 1 hour
Routine: 4 hours
Times are after each specimen is drawn
Test Schedule
Daily
Sample Stability
Refrigerated: 14 days
Frozen: 30 days
Specimen Processing
Spin completely clotted SST tube for 10 minutes. Store tubes in refrigerator until testing can be done.
Method
Chemiluminescent microparticle immunoassay (CMIA)
Reference Ranges
Cortisol Pre-Stimulation ug/dL
Cortisol AM (4-9AM): 3.7-19.4
Cortisol Random: 2.9-17.3
Critical Value: None
Reporting Limit
Measuring Range: 1.0 - 59.8 ug/dL
Reporting Range: 1.0 -119.6 ug/dL (Automated 1:2 Dilution)
Synonyms
Cortisol Stimulation (3 samples)
Test Components
Cortisol ACTH Stimulation, Baseline, ug/dL; Date/Time Collected;
Cortisol ACTH Stimulation, 30 min, ug/dL; Date/Time Collected;
Cortisol ACTH Stimulation, 60 min, ug/dL; Date/Time Collected.
CPT Codes
82533 × 2