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Test Code LAB4004 CORTISOL ACTH STIMULATION (BASELINE, 30MIN, 60MIN)

Specimen Type

Preferred Sample: Lithium Heparin Plasma (PST, Mint Green Tube, Dark Green tube)

Alternate Sample: Serum (SST, Gold, Corvac, Tiger, Red Top Tube)

 

Specimen Volume

1 mL for each timed sample

Minimum Volume

0.5 mL for each timed sample

Turnaround Time

STAT: 1 hour
Routine: 4 hours

Test Schedule

Daily

Sample Stability

Refrigerated: 14 days

Frozen: 30 days

 

Method

Chemiluminescent microparticle immunoassay (CMIA)

Reference Ranges

Cortisol Pre-Stimulation ug/dL 

        Cortisol AM (4-9AM): 3.7-19.4

        Cortisol Random: 2.9-17.3

Critical Value: None

Reporting Limit

Reporting Range: 1.0 -119.6 ug/dL

Synonyms

Cortisol Stimulation (3 samples)

CPT Codes

82533 × 2

Collection Instructions

The baseline cortisol specimen should be collected prior the administration of the cosyntropin medication.

The 30 min cortisol sample should be collected 30 minutes after the administration of the cosyntropin medication.

The 60 min cortisol sample should be collected 60 minutes after the administration of the cosyntropin medication.

See also: Cortisol Collection Job Aid in PPM

 

Specimen Processing

Spin completely clotted SST tube for 10 minutes. Store tubes in refrigerator until testing can be done.

Test Components

Cortisol ACTH Stimulation, Baseline, ug/dL; Date/Time Collected;

Cortisol ACTH Stimulation, 30 min, ug/dL; Date/Time Collected;

Cortisol ACTH Stimulation, 60 min, ug/dL; Date/Time Collected.