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Test Code LAB4076 DISSEMINATED INTRAVASCULAR COAGULATION SCREEN

Important Note

Blue top tube must be filled completely to maintain the correct anticoagulant / blood ratio.

Specimen Type

Blue top tube (buffered 3.2% liquid sodium citrate) filled to the indicator with whole blood or Sodium Citrate Plasma removed from cells.

Collection Instructions

Blue top must be filled appropriately for tube size. Volume of blood to anticoagulant in tube is critical. Lavender top tube must be mixed properly at time of collection to ensure specimen does not clot.

Specimen Volume

3mL Blue top (3.2% Sodium Citrate), or
5mL Blue top (3.2% Sodium Citrate), or
1mL Blue top (3.2% Sodium Citrate) MiniCollect vial, or
500uL (minimum) Sodium Citrate Plasma spun and removed from cells.

Minimum Volume

500uL EDTA  microtainer + 1mL Blue top (3.2% Sodium Citrate) MiniCollect vial.

 

Turnaround Time

STAT: 1 hour
Routine: 4 hours

Test Schedule

Daily

Sample Stability

Non-heparin Patients: 4 hours
Patients on heparin: 1 hour. 
Frozen samples are stable for 2 weeks at -20C or 12 months at -70C.

Specimen Processing

Lavender top: Do not spin, EDTA whole blood.

Blue top: Assay of non-heparinized patients must be performed within 4 hours of collection. Assays on specimens suspected to contain unfractionated heparin therapy should be tested within 1 hour of collection.  If time interval between drawing and testing exceeds 1 hour, centrifuge specimen, separate plasma, centrifuge, separate into plastic tube and freeze at -20C or less. Frozen samples are stable for 2 weeks at -20C or 12 months at -70C.

Method

Electromechanical, Microscopy

Reference Ranges

Protime: 11.6-14.7 sec

INR: 0.9-1.2

    Usual oral anitcoagulation range 2.0-3.0

    High-level oral anticoagulation range 2.5-3.5

PTT: 23.5-34.6 sec

Fibrinogen: 212.0 – 451.0 mg/dL

D-Dimer,Qnt: < 0.50 µg/mL FEU

Values of less than or equal to 0.50 µg/mL FEU have a negative predictive value of 98% for VTE (PE and DVT) when the Wells pretest probability is low or moderate. Results of the D-Dimer test should always be interpreted in conjunction with the patient’s medical history and clinical presentation. A very low percentage of patients with DVT may yield results below the cut-off of 0.50 µg/mL FEU  Wells,PS; et al. Use of a Clinical Model for Safe Management of Patients with Suspected PE

Platelet Count K/uL

    0-3 days 250-450

    3-9 days 200-400

    9-30 days 250-450

    1-6 mo 300-750

    6 mo-2 yrs 250-600

    2-8 yrs 250-550

    8-12 yrs 200-450

    12-18 yrs 150-450

    18 yrs+ 150-400

Fragmented Cells:  Absent

Synonyms

DIC Screen

Test Components

Protime, sec;

INR;

PTT, sec;

Fibrinogen, mg/dL;

D-Dimer, mg/L FEU;

Platelet Count, K/uL;

Fragmented Cells

CPT Codes

85610, 85730, 85384, 85380

Rejection Criteria

Severely hemolyzed or clotted samples, inappropriately filled liquid blue top tubes, specimens not processed within 4 hours.