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Test Code LAB4156 PLATELET P2Y12 FUNCTION

Important Note

Draw 2 special 'vacuette' blue top 2mL tubes (note: not 2.7 mL tubes). Specimens must be hand carried to the lab. Do not send via pneumatic tube system.

Specimen Type

Sodium Citrate whole blood in 2 special Blue top with White rim 2 mL tubes.

Collection Instructions

Draw using a 21 gauge or larger needle. Collect a 1.8 mL blue top discard tube or if drawing from a line, draw & discard 5 mL. Then draw two special blue/white top tube with 2 mL to the black line. Do NOT overfill. Gently invert 5 times.If drawing for other tests, draw these samples last.

Specimen Volume

4 mL

Minimum Volume

2 mL

Turnaround Time

STAT: 1 hour
Routine: 4 hours

Test Schedule

Daily

Sample Stability

Room Temp: 4 hours

Specimen Processing

Do not refrigerate or centrifuge. Transport immediately to Lab at room temperature. Do NOT send specimens in the pneumatic tube system.

Method

VerifyNow Platelet Aggregation for P2Y12 Inhibition

Reference Ranges

Platelet Function P2Y12, PRU

Concordance of PRU and % Inhibition Results for the VerifyNow P2Y12 Test PRU Results from Clopidogrel-Dosed Patient Samples with a Low Percent Inhibition.

In an analysis of the 10,375 sample dataset from a recent clinical trial, PRU and % inhibition results reported by the VerifyNow Instrument were analyzed. Receiver operating characteristic (ROC) curve analysis was used to identify the PRU threshold when sensitivity was equal to specificity for detecting whether % inhibition was above or below the defined threshold. The PRU thresholds that provided the best prediction of % inhibition above or below thresholds of 10%, 20%, 30%, 40%, 50% and 60% were determined and are shown below.

Inhibition Threshold                    PRU Threshold               ROC AUC

   10%                                                  259                               0.91

   20%                                                  237                               0.93

   30%                                                  214                               0.95

   40%                                                  187                               0.97

   50%                                                  159                               0.98

   60%                                                  131                               0.99

The results demonstrated a very high statistically significant (p < 0.001) area under the ROC curve, suggesting that the PRU results have excellent ability to discriminate a % inhibition result above or below the specified threshold.

Synonyms

VerifyNowPlavix; P2Y12; Plavix (clopidogrel) Inhibition; ADP Platelet Inhibition

Test Components

Platelet Function P2Y12, PRU

CPT Codes

85576 × 2

Test Limitations

Do NOT transport specimens via the pneumatic tube system.