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Test Code LAB4226 VITAMIN D, 25 HYDROXY

Important Note

It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at leat 72 hours prior to the collection of sample.

Specimen Type

Preferred Sample: Lithium Heparin Plasma (Green/Mint Top)
Alternative Sample: Serum (SST, Gold, Tiger, Red Top NO GEL)

Specimen Volume

1 mL

Minimum Volume

0.5 mL

Microtainer - 0.2mL

Turnaround Time

STAT: 1 hour
Routine: 4 hours

Test Schedule

Daily

Sample Stability

Refrigerated: 12 days
Frozen: 12 months

Specimen Processing

Allow specimen to clot completely. Separate serum from cells ASAP and transport refrigerated. If red top tube is collected, separate serum from cells ASAP and place in separate plastic tube and cap immediately. Store and transport refrigerated.

Method

Immunoassay

Reference Ranges

Vitamin D, 25 Hydroxy

>29.0 ng/mL

 

Toxicity is possible at 80 ng/mL or greater (0-18 years) or at 150 ng/mL or greater (19+ years). If toxicity is a clinical concern, recommend testing by LC-MS/MS.

Synonyms

 CALCIFEDIOL, 25 HYDROXYCALCIFEROL, CHOLECALCIFEROL METABOLITE, D3 METABOLITE, VDOHKH, LAB4226

CPT Codes

82306

Rejection Criteria

Grossly hemolyzed, grossly lipemic, or jaundiced samples

Reporting Limits

Measuring and reporting ranges: 3.5-154.2 ng/mL