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HelpTest Code LAB4257 Anti-Xa Assay, UFH
Specimen Type
Blue top tube (buffered 3.2% liquid sodium citrate) filled to the indicator with whole blood or Sodium Citrate Plasma removed from cells.
Collection Instructions
Fill a Blue Top Container to the Indicator mark on the tube. Overfilled and underfilled tubes are not acceptable.
Specimen Volume
3mL Blue top (3.2% Sodium Citrate)
5mL Blue top (3.2% Sodium Citrate)
1mL Blue top (3.2% Sodium Citrate)
500uL (minimum) Sodium Citrate Plasma spun and removed from cells.
Turnaround Time
STAT: 1 hour
Routine: 4 hours
Test Schedule
Daily
Sample Stability
Room temp: 1 hour unspun. Platelet poor plasma is stable for two hours.
Frozen: 2 weeks
Specimen Processing
Samples should be centrifuged and run within one hour. If time interval between drawing and testing exceeds 1 hour, process sample to create platelet poor plasma (centrifuge specimen, separate plasma, centrifuge again, separate into plastic tube) and freeze at -20C. Platelet poor plasma is stable refrigertaed for 2 hours, Frozen (-20 C) for 2 weeks, or Frozen (-70 C) for 12 months.
Method
Anti-Xa Chromogenic
Reference Ranges
Anti-Xa, UFH: ≤0.10 IU/mL
Critical Range: >1.0m IU/mL
Synonyms
Unfractionated Heparin, Anti-Xa; Heparin
CPT Codes
85520
Rejection Criteria
Severely hemolyzed specimens, clotted samples. Overfilled and underfilled tubes are not acceptable