Collection Instructions

Urine Specimen Collection Steps:

1.          First void urine specimens (preferred) must be collected in a sterile plastic preservative free specimen collection cup. Urine must be transferred to a BD Molecular Urine Sample Buffer Tube (yellow top) IMMEDIATELY after collection.

2.          Uncap the BD Molecular Urine Sample Buffer Tube and the urine specimen cup, taking care not to contaminate the contents or the outside of the tube.

3.          Immediately after collection, use the graduated transfer pipette to mix the urine specimen gently in the collection cup and transfer approximately 2 ml into the BD Molecular Urine Sample Buffer Tube – NOTE: Use the graduations on the transfer pipette as a guide.

4.          Use the viewing window on the BD Molecular Urine Sample Buffer Tube label to ensure urine specimens was added to the tube.

5.          Discard the transfer pipette in a biohazard waste container – the transfer pipette is intended for use with a single specimen.

6.          Tighten the cap securely on the BD Molecular Urine Sample Buffer tube.

7.          Invert the BD Molecular Urine Sample Buffer Tube 3 to 4 times to ensure that the specimen and reagent are well mixed.

8.          Label the BD Molecular Urine Sample Buffer Tube with the patient identification and date/time collected. Do not obscure the barcodes on the tube.

 Vaginal Specimen Collection Steps:

·         Collect swab prior to pelvic, speculum, or bimanual exam.

·         No lubricant is used for the sample technique.

·         Do not collect at the posterior fornix

1.       Gently slide the swab 2 inches (5 cm) into the vagina. If the swab does not slide easily, gently rotate the swab as you push. If it is still difficult, do not attempt to continue.

2.       Rotate the swab for 10 to 15 seconds.

3.       Withdraw the swab without touching the skin outside the vagina.

a.        Note: Specimen must be transferred immediately into the Swab Buffer tube with the green cap.

4.       Unscrew the cap of the BD Molecular Swab Sample Buffer Tube, taking care not to contaminate the contents or the outside of the tube.

5.       To transfer the sample, fully insert the swab into the tube so the tip is at the bottom after carefully removing the green cap so it can be used later to recap the specimen.

6.       Break the shaft at the score mark, taking care to avoid splashing.

7.       Tightly recap the tube.

8.       Label the tube with patient information, date and time collected, taking care to not cover all of the barcodes.