Collection Instructions

Urine Specimen (Dirty) Collection Instructions:

1. The patient should not have urinated for at least 1 hour prior to specimen collection. Patient should NOT cleanse genital area prior to collecting the specimen.
2. Direct patient to provide first-catch urine (approximately 20 to 50 mL of the initial urine stream) into a urine collection cup free of any preservatives.
3. Transfer approximately 7 mL of urine into the Xpert Urine Transport Reagent tube (yellow top) using the disposable transfer pipette up to the black dashed line (minimum volume 3mL).
4. Tighten the cap securely on the BD Molecular Urine Sample Buffer tube.
5.  Invert the reagent tube 3 to 4 times to ensure that the specimen and reagent are well mixed.
6. Label tube with the patient information.
7. Ship to laboratory at room temperature.

 Vaginal Specimen Collection Instructions:

·         Collect swab prior to pelvic, speculum, or bimanual exam.

·         No lubricant is used for the sample collection.

·         Do not collect at the posterior fornix

1. Discard the large cleaning swab. Partially peel open the FLOQSwab in collection kit. Carefully insert the FLOQSwab 2 inches (5 cm) into the vagina.
2. Rotate the swab for 10 to 30 seconds. Ensure the swab touches the wall of the vagina.
3. Specimen must be transferred immediately into the Xpert Swab Specimen Collection Tube with the pink cap. Insert swab into collection tube and break the shaft at the score mark.
4. Tightly recap the tube. Invert or gently shake tube 3-4 times.
5.  Label the tube with patient information.
6. Ship to laboratory at room temperature.

Cervix/Endocervical Specimen Collection Instructions:

1. Partially open the large cleaning swab. Use the large cleaning swab to remove excess mucus from the cervical os and surround mucosa. Discard the large cleaning swab.
2. Partially peel open the FLOQSwab in collection kit. Endocervical collection- carefully insert the swab into the endocervical canal, gently rotate the swab for 10 to 30 seconds. Cervix collection- swab the cervix and surrounding mucosa. Specimen must be transferred immediately into the Xpert Swab Specimen Collection Tube (pink top).
3. Unscrew the cap of the Xpert Swab Specimen Collection Tube (pink top).
4. Fully insert the swab into the Xpert Swab Specimen Collection Tube (pink top) so the tip is at the bottom after carefully removing cap so it can be used later to recap the specimen.
5. Break the shaft at the score mark, taking care to avoid splashing. If the contents of the tube are spilled, use a new collection kit.
6. Tightly recap the tube. Invert or gently shake tube 3-4 times.
7. Label the tube with patient information.
8. Ship to Laboratory at Room Temperature.