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Test Code MUCN2 Mucin 2, Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required, order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Specimen Type: Tissue

Supplies: Immunostain Technical Only Envelope (T693)

Container/Tube: Immunostain Technical Only Envelope

Preferred:

-Formalin-fixed, paraffin-embedded tissue block

OR

-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: None


Forms

If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Secondary ID

605116

Useful For

Aiding in subtyping intraductal papillary mucinous neoplasms

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOA IHC Additional, Tech Only No No
IHTOI IHC Initial, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

Mucin 2 IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Wet/frozen tissue 
Cytology smears 
Nonformalin fixed tissue 
Nonparaffin embedded tissue 
Noncharged slides 
ProbeOn slides 
Snowcoat slides 		 Reject 

Clinical Information

Mucin 2 (MUC2) is a 520 kDa glycoprotein belonging to the family of secretory mucins. MUC2 is expressed in the cytoplasm of goblet cells. An immunopanel consisting of MUC1, MUC2, MUC5AC, and MUC6 is useful in subtyping intraductal papillary mucinous neoplasms.

Interpretation

This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.

 

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks.

 

The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.

 

Best practices for storage of positively charged slides:

-Minimize time slides are stored after being unpackaged

-Limit exposure to high humidity and heat

-Minimize exposure to plastics

Clinical Reference

1. Lau S, Weiss L, Chu P. Differential expression of MUC1, MUC2, and MUC5AC in carcinomas of various sites.  Am J Clin Pathol. 2004;122:61-69. doi:10.1309/9R66-73QE-C06D-86Y4

2. Pezhouh M, Park J. Gastric pyloric gland adenoma. Arch Pathol Lab Med. 2015;139:823-826. doi:10.5858/arpa.2013-0613-RS

3. Kwak H, Liu X, Allende D, Pai RK, Hart J, Xiao SY. Interobserver variability in intraductal papillary mucinous neoplasm subtypes and application of their mucin immunoprofiles. Mod Pathol. 2016;29:977-984. doi:10.1038/modpathol.2016.93

4. Kim D, Shin N, Kim G, et al. Mucin expression in gastric cancer. Reappraisal of Its Clinicopathologic and Prognostic Significance. Arch Pathol Lab Med. 2013;137:1047-1053. doi.org/10.5858/arpa.2012-0193-OA

5. Castellano-Megias V, Ibarrola-de Andres C, Lopez-Alonso G, Colina-Ruizdelgado F. Pathological features and diagnosis of intraductal papillary mucinous neoplasm of the pancreas. World J Gastrointest Oncol. 2014;6(9):311-324. doi:10.4251/wjgo.v6.i9.311

6. Horinouchi M, Nagata K, Nakamura A, et al. Expression of different glycoforms of membrane mucin (MUC1) and secretory mucin (MUC2, MUC5AC and MUC6) in pancreatic neoplasms. Acta Histochem Cytochem. 2003;36(5):443-453. doi:10.1267/ahc.36.443

7. Magaki S, Hojat SA, Wei B, So A, Yong WH. An Introduction to the Performance of Immunohistochemistry. Methods Mol Biol. 2019;1897:289-298. doi:10.1007/978-1-4939-8935-5_25

Method Description

Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Specimen Retention Time

Until staining is complete

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MUCN2 Mucin 2 IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
605117 Mucin 2 IHC, Tech Only Bill only; no result